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Using the field plot test, set up four treatments, one for each species, three replicates for each species, and randomly arrange 36 tobacco plants in each plot. Each plant used 5 g of pure nitrogen as the base fertilizer. 1g was used as seedling fertilizer in 3 applications, and 3g was used as a topdressing fertilizer in the trenches for one-time application of N. The mass ratio of Pz05 to Kz0 was 1:1:2. Other measures and management were conducted in accordance with high-quality tobacco cultivation and management measures.
It has been considered that the chlorophyll meter reading has a very good linear relationship with the chlorophyll content of different leaf positions and maturity, and the correlation can be fitted with a force of y=ax+b. The sap value can be used to express the chlorophyll content. The level of chlorophyll spad in leaves of four flue-cured tobacco cultivars K326, Dahua Dajing, NC82 and Liaoyan No. 13 was determined by SPAD-502 chlorophyll meter. feature.
Flue-cured tobacco was started on the 7th week after transplanting ((0d), 6 consecutive times for every Sd ((0, 5, 10, 15, 20, 25d)) and 5 fixed cigarettes were taken from each experimental plot. Each cigarette was the same. One leaf (10th functional leaf removed from the bottom of the foot) was selected for listing. The SPAD-502 chlorophyll meter was used to measure the chlorophyll spad values ​​at the leaf base, mid leaf, and tip 3 positions. The relative change in pad value was calculated. Rate.spad value relative rate of change = (each measured pad value / first measured pad value) X100%.
The value of the padding value of different flue-cured tobacco leaves in the same location measured by chlorophyll meter was influenced by different parts, and it was also related to the time measured by the instrument. In the time range measured by chlorophyll meter, the leaf chlorophyll spad values ​​of K326, Dahua Jinjin, Liaoyan No. 13 and NC82 were the smallest at the leaf base and the largest at the tip of the leaf. The sap value of chlorophyll at the site of the determination was significantly different or extremely significant; in the last three determinations, there was no significant difference in the sap value measured by the chlorophyll meter at the same leaf position between the leaves of S. grandiflora and Liaoyan 13 and the leaf color was uniform.
The chlorophyll content is an important reference parameter in the important components of the pigments in the tobacco leaves and the judgment of the depth of the color. The chlorophyll content not only affects the yield of tobacco leaves but also has a large relationship for the determination of the harvest season. The aroma of the leaf and the amount of gas are also related. Therefore, in the study of tobacco leaves, the research on the chlorophyll meter's padding value has been paid close attention. At the same time, because there are many factors affecting the chlorophyll content, there is a certain degree of extensiveness in the research field.